Publicaciones sobre regulación del metabolismo secundario

Martínez-Burgo Y, Santos-Aberturas J, Rodríguez-García A, Barreales EG, Tormo JR, Truman A, Reyes F, Aparicio JF, Liras P (2019). Activation of secondary metabolite gene clusters in Streptomyces clavuligerus by the PimM regulator of Streptomyces natalensis. Front. Microbiol. 10: 580. Pubmed

Barreales EG, Payero TD, de Pedro A, Aparicio JF (2018). Phosphate effect on filipin production and morphological differentiation in Streptomyces filipinensis and the role of the PhoP transcription factor. PloS One 13: e0208278. Pubmed

Barreales EG, Vicente CM, de Pedro A, Santos-Aberturas J, Aparicio JF (2018). Promoter engineering reveals the importance of heptameric direct repeats for DNA-binding by SARP-LAL regulators in Streptomyces natalensis. Appl. Environ. Microbiol.  84: e00246-18. Pubmed

Aparicio JF, Barreales EG, Payero TD, Vicente CM, de Pedro A, Santos-Aberturas J. (2016).   Biotechnological production and application of the antibiotic pimaricin: biosynthesis and its regulation. Appl. Microbiol. Biotechnol. 100: 61-78. Pubmed

Payero TD, Vicente CM, Rumbero A, Barreales EG, Santos-Aberturas J, de Pedro A, Aparicio JF. (2015). Functional analysis of filipin tailoring genes from Streptomyces filipinensis reveals alternative routes in filipin III biosynthesis and yields bioactive derivatives. Microb. Cell Fact. 14: 114. Pubmed

Vicente CM, Payero TD, Santos-Aberturas J, Barreales EG, de Pedro A, Aparicio JF. (2015). Pathway-specific regulation revisited: cross-regulation of multiple disparate gene clusters by PAS-LuxR transcriptional regulators. Appl. Microbiol. Biotechnol. 99: 5123-5135. Pubmed

Vicente CM, Santos-Aberturas J, Payero TD, Barreales EG, de Pedro A, Aparicio JF. (2014). PAS-LuxR transcriptional control of filipin biosynthesis in S. avermitilis. Appl. Microbiol. Biotechnol.  98: 9311-9324. Pubmed

Beites T, Rodríguez-García A, Santos-Beneit F, Moradas-Ferreira P, Aparicio JF, Mendes MV. (2014). Genome-wide analysis of the regulation of pimaricin production in Streptomyces natalensis by reactive oxygen species. Appl. Microbiol. Biotechnol.  98: 2231-2241. Pubmed

Santos-Aberturas J, Vicente CM, Payero TD, Martín-Sánchez L, Cañibano C, Martín JF, Aparicio JF. (2012). Hierarchical control on polyene macrolide biosynthesis: PimR modulates pimaricin production via the PAS-LuxR transcriptional activator PimM. PloS One 7: e38536. Pubmed

Barreiro C, Prieto C, Sola-Landa A, Solera E, Martínez-Castro M, Pérez-Redondo MR, García Estrada C, Aparicio JF, Fernández-Martínez LT, Santos-Aberturas J, Salehi-Najafabadi Z, Rodríguez-García A, Tauch A, Martín JF. (2012). Draft Genome of Streptomyces tsukubaensis NRRL 18488, the producer of the clinically important immunosuppressant tacrolimus (FK506). J. Bacteriol. 194: 3756-3757. Pubmed

Guerra SM, Rodríguez-García A, Santos-Aberturas J, Vicente CM, Payero TD,  Martín JF, Aparicio JF. (2012). LAL Regulators SCO0877 and SCO7173 as Pleiotropic Modulators of Phosphate Starvation Response and Actinorhodin Biosynthesis in Streptomyces coelicolor. PloS One 7: e31475. Pubmed

Santos-Aberturas J, Payero TD, Vicente CM, Guerra SM, Cañibano C, Martín JF, Aparicio JF. (2011). Functional conservation of PAS-LuxR transcriptional regulators in polyene macrolide biosynthesis. Metab. Eng. 13: 756-767. Pubmed

Santos-Aberturas J, Vicente CM, Guerra SM, Payero TD, Martín JF, Aparicio JF. (2011). Molecular control of polyene macrolide biosynthesis: direct binding of the regulator PimM to eight promoters of pimaricin genes and identification of binding boxes. J. Biol. Chem. 286: 9150-9161. Pubmed

Kells PM, Ouellet H, Santos-Aberturas J, Aparicio JF, Podust LM. (2010). Structure of cytochrome P450 PimD suggests epoxidation of the polyene macrolide pimaricin occurs via a hydroperoxoferric intermediate. Chem. Biol. 17: 841-851. Pubmed

Vicente CM, Santos-Aberturas J, Guerra SM, Payero TD, Martín JF, Aparicio JF. (2009). PimT, an amino acid exporter controls polyene production via secretion of the quorum sensing pimaricin-inducer PI-factor in Streptomyces natalensis. Microb. Cell Fact. 8: 33. Pubmed

Martín JF, Aparicio JF. (2009). Enzymology of the polyenes pimaricin and candicidin biosynthesis. Meth. Enzymol. 459: 215-242. Pubmed

Aparicio JF, Martín JF. (2008). Microbial cholesterol oxidases: Bioconversion enzymes or signal proteins?. Mol. Biosyst. 4: 804-809. Pubmed

Caffrey P, Aparicio JF, Malpartida F, Zotchev SB. (2008). Biosynthetic Engineering of Polyene Macrolides Towards Generation of Improved Antifungal and Antiparasitic Agents. Curr. Top. Med. Chem. 8: 639-653. Pubmed

Antón N, Santos-Aberturas J, Mendes MV, Guerra SM, Martín JF, Aparicio JF. (2007). PimM, a PAS domain positive regulator of pimaricin biosynthesis in Streptomyces natalensis. Microbiology SGM 153: 3174-3183. Pubmed

Mendes MV, Recio E, Antón N, Guerra SM, Santos-Aberturas J, Martín JF, Aparicio JF. (2007). Cholesterol oxidases act as signalling proteins for the biosynthesis of the polyene macrolide pimaricin. Chem. Biol. 14: 279-290. Pubmed

Mendes MV, Tunca S, Antón N, Recio E, Sola-Landa A, Aparicio JF, Martín JF. (2007). The two-component phoR-phoP system of Streptomyces natalensis: Inactivation or deletion of phoP reduces the negative phosphate regulation of pimaricin biosynthesis. Metab. Eng. 9: 217-227. Pubmed

Recio E, Aparicio JF, Rumbero A, Martín JF. (2006). Glycerol, ethylene glycol and propanediol elicit pimaricin biosynthesis in the PI-factor defective strain Streptomyces natalensis npi287 and increase polyene production in several wild type actinomycetes. Microbiology SGM 152: 3147-3156. Pubmed

Enríquez LL, Mendes MV, Antón N, Tunca S,  Guerra SM, Martín JF, Aparicio JF. (2006). An efficient gene transfer system for the pimaricin producer Streptomyces natalensis. FEMS Microbiol. Lett. 257: 312-318. Pubmed

Aparicio JF. (2005). Generating novel polyene antifungal drugs. Chem. Biol. 12: 509-510. Pubmed

Mendes MV, Antón N, Martín JF, Aparicio JF. (2005). Characterization of the polyene macrolide P450 epoxidase from Streptomyces natalensis that converts deepoxypimaricin into pimaricin. Biochem. J. 386: 57-62. Pubmed

Recio E,  Colinas A, Rumbero A, Aparicio JF, Martín JF. (2004). PI factor, a novel type quorum-sensing inducer elicits pimaricin production in Streptomyces natalensis. J. Biol. Chem. 279: 41586-41593. Pubmed

Antón N, Mendes MV, Martín JF, Aparicio JF. (2004). Identification of PimR as a positive regulator of pimaricin biosynthesis in Streptomyces natalensis. J. Bacteriol. 186: 2567-2575. Pubmed

Fernández M. Cuadrado Y, Aparicio JF, Martín JF. (2004). Role of homoserine and threonine pathway intermediates as precursors for the biosynthesis of aminoethoxyvinylglycine in Streptomyces sp. NRRL 5331. Microbiology SGM 150: 1467-1474. Pubmed

Aparicio JF, Mendes MV, Antón N, Recio E, Martín JF. (2004). Polyene macrolide antibiotic biosynthesis. Curr. Med. Chem. 11: 1645-1656. Pubmed

Cuadrado Y, Fernández M, Recio E, Aparicio JF, Martín JF. (2004). Characterization of the ask-asd operon in aminoethoxyvinylglycine-producing Streptomyces sp. NRRL 5331. Appl. Microbiol. Biotechnol. 64: 228-236. Pubmed

Hijarrubia MJ, Aparicio JF, Martín JF. (2003). Domain Structure Characterization of the Multifunctional α-Aminoadipate Reductase from Penicillium chrysogenum by Limited Proteolysis: Activation of α Aminoadipate does not require the PCP Box or the Reduction Domain. J. Biol. Chem. 278: 8250-8256. Pubmed

Aparicio JF, Caffrey P, Gil JA, Zotchev SB. (2003). Polyene antibiotic biosynthesis gene clusters. Appl. Microbiol. Biotechnol. 61: 179-188. Pubmed

Hijarrubia MJ, Aparicio JF, Martín JF. (2002). Nitrate regulation of α-aminoadipate reductase formation and lysine inhibition of its activity in Penicillium chrysogenum and Acremonium chrysogenum. Appl. Microbiol. Biotechnol. 59: 270-277. Pubmed

Mendes MV, Recio E, Fouces R, Luiten R, Martín JF, Aparicio JF. (2001). Engineered biosynthesis of novel polyenes: A pimaricin derivative produced by targeted gene disruption in Streptomyces natalensis. Chem. Biol. 8: 635-644. Pubmed

Aparicio JF, Fouces R, Mendes MV, Olivera N, Martín JF. (2000). A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis. Chem. Biol. 7: 895-905. Pubmed